Application

Research CategoryApoptosis & CancerMetabolism

Western blot: 1 µg/ml for 2 hours at RT.

Immunoprecipitation: 2 µg/mg of protein lysate Immunohistochemistry (frozen and paraffin): 2-4 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT.

Optimal working dilutions must be determined by end user.

Research Sub CategoryCaspasesEnzymes & Biochemistry

Detect Caspase 3 using this Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03 validated for use in IP, WB, IH(P).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: large subunit & proform

Recombinant full-length human caspase-3 protein

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-440; 04-1088

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.

Specificity

Recognizes a protein of 32 kDa, identified as the Caspase-3 protein. Highly specific to Caspase-3 and shows no cross-reaction with other caspase family members. Caspase-3 is ubiquitously expressed and like other caspases is synthesized as an inactive proenzyme. Upon activation, caspase-3 is cleaved generating two subunits of 17 kDa and 12 kDa respectively. Caspase-3 has been implicated in the induction of apoptosis. Activation of caspase-3 occurs in response to a variety of apoptotic inducers, including Fas-mediated apoptosis.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.